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. 2017 Jul 10;18(7):1474. doi: 10.3390/ijms18071474

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Localisation of CD15s on the surface of adherent SEBTA-001 cells at the site of adhesion. Confocal image of green fluorescently-labelled adherent brain to lung metastatic cancer cells (SEBTA-001) on a monolayer of activated hCMEC/D3 cells (blue). (A) Expression of CD15s (red) (white arrows) was seen on the edges of adherent cells SEBTA-001 (green) at the site of adhesion on brain endothelial cells; (B) expression of CD15s (red) (white arrows) was observed on surface of adherent cancer cells SEBTA-001 (green) and CD62E (purple) (white arrows) was seen on the monolayer of activated brain endothelial cells (hCMEC/D3) at adhesion site of cancer cell–brain endothelium. Scale bar = 20 µm.