Figure 4.

Lactate dehydrogenase (LDH) release of chondrocyte pellets cultured in an OA-model. Patient-derived OA chondrocyte pellets were cultured in media supplemented with or without rapamycin, the inflammatory cytokine interleukin (IL)-1β or rapamycin in combination with IL-1β for 14 days. Chondrocyte pellets cultured with culture media alone are indicated as control. Supernatants were collected on indicated time points and released LDH was measured. Experiments were performed in triplicates and results are shown as mean ± SEM versus control. p < 0.05 was considered significant (*) and p < 0.01 was considered highly significant (**). Exact values were given when p > 0.05.