Figure 4.

Effects of LA on pulmonary thrombosis and fluorescein sodium-induced platelet thrombus formation in the mesenteric microvessels of mice. (A) Mice (male, 5–6 weeks old) were intraperitoneally administered with DMSO (solvent control) or LA (1.8 and 3.6 mg/kg) for 30 min. ADP (1.4 g/kg) was injected in the tail vein to induce acute pulmonary thrombosis. The survival rate (bottom panel) was determined at 1 and 24 h after ADP administration, and pulmonary thrombosis (top panel, arrows) was observed by staining lung tissue sections with hematoxylin-eosin. Scale bar: 200 μm. The survival rate was evaluated using the Kaplan–Meier survival method (n = 8). *** p < 0.001, compared with the sham-operated group. ^# p < 0.05, compared with the DMSO group; (B) Mice received an intravenous bolus of DMSO or LA (1.8 and 3.6 mg/kg), and the mesenteric venules were irradiated to induce microthrombus formation. Data are presented as the mean ± SEM (n = 8). ** p < 0.01 and *** p < 0.001, compared with the DMSO group.