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. 2017 Jul 18;18(7):1563. doi: 10.3390/ijms18071563

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogenetic relationship of transcripts putatively encoding lipocalins (Lip) identified in the Haller’s organ spf (contig 84287) and the Illumina 1st and 4th leg transcriptomes (contigs 42763, 1st legs; contig 466, 4th legs) of unfed, virgin adult male Dermacentor variabilis with lipocalins from Amblyomma triste, Ixodes ricinus, Rhipicephalus microplus and Rattus norvegicus. The phylogenetic tree shows the branch relation of chemosensory lipocalins (red branch) with non-chemosensory lipocalins. Tick putative lipocalins are highlighted with a blue branch color. Acronyms are as follows: first letter of the genus and species (Amblyomma triste, At; Ixodes ricinus, Ir; Rattus norvegicus, Rn; Rhipicephalus microplus, Rm) followed by the protein name (OBlip or Lip). Putative lipocalin transcripts are boxed. The tree was constructed using Maximum likelihood phylogenetic analysis and bootstrapping set to 500 iterations. Branch values listed are bootstrap percentages (percent confidence), scale set to 20%. A comprehensive list of acronyms and associated GenBank accession numbers are listed in Appendix A.