Figure 3.

Phylogenetic relationship of transcripts putatively encoding G-protein α subunits (Gα), one transcript identified in the Haller’s organ spf transcriptome (contig 13937) and transcripts found common in the Illumina 1st and Illumina 4th leg transcriptomes (contigs 14072 and 46297, 1st legs; contigs 2423 and 13329, 4th legs) of unfed, virgin adult male Dermacentor variabilis with Gα subunits of known clade annotation from Caenorhabditis elegans and insects. The phylogenetic tree shows four clades, each represented by the following branch colors: red = Gαi/o clade; green = Gαq clade; purple = Gαq12/13 clade; blue = Gαs clade. Acronyms are as follows: first letter of the genus and species (Anopheles aquasalis, Aa; Anopheles gambiae, Ag; Drosophila melanogaster, Dm; Caenorhabditis elegans, Ce) followed by the protein name (Gα) and the letter/number of the associated clade. Putative Gα subunit transcripts are boxed. The tree was constructed using Maximum likelihood phylogenetic analysis and bootstrapping set to 500 iterations. Branch values listed are bootstrap percentages (percent confidence), scale set to 20%. A comprehensive list of acronyms and associated GenBank accession numbers are listed in Appendix A.