Table 1.
The 50 most abundant transcripts from the unfed, virgin adult male Dermacentor variabilis Illumina 1st leg transcriptome.
| Contig | Unique Reads | Accession No. | Best Match (Lowest e-Value) to UniprotKB Database | Organism | e-Value | Conserved Domain(s) a | Putative Function |
|---|---|---|---|---|---|---|---|
| 77381 | 1709234 | EAW18142 | Hypothetical protein | Neosartorya fischeri | 6E+00 | None | Unknown |
| 04832 | 439971 | EEC05627 | Putative titin | Ixodes scapularis | 00E+00 | I-set | Myofibril scaffold |
| 00022 | 410660 | JAC21803 | Putative troponin T skeletal muscle | Rhipicephalus appendiculatus | 00E+00 | Troponin | Skeletal muscle contraction |
| 00073 | 278198 | AAP79880 | Actin | Rhipicephalus microplus | 00E+00 | None | Skeletal muscle contraction |
| 00084 | 181667 | AGH19694 | Cytochrome oxidase subunit 1 | Dermacentor nitens | 00E+00 | None | Aerobic metabolism |
| 03069 | 167176 | BAK26392 | Ryanodine receptor | Tetranychus urticae | 00E+00 | Ins145_P3_rec, MIR | Intracellular cation transport |
| 00151 | 137793 | JAC30240 | Uncharacterized protein | Amblyomma triste | 00E+00 | PDZ | Unknown |
| 00696 | 137289 | JAC22297 | Putative titin | Amblyomma cajennense | 00E+00 | I-set, fn3 | Myofibril scaffold |
| 13661 | 118729 | AAD23988 | Beta-actin | Tupaia belangeri | 2E-79 | None | Cytoskeleton |
| 00033 | 112728 | ABB89211 | Troponin I | Rhipicephalus haemaphysaloides | 8E-49 | None | Skeletal muscle contraction |
| 00545 | 109190 | XP_003739692 | Uncharacterized protein | Metaseiulus occidentalis | 6E-17 | None | Unknown |
| 18048 | 94602 | CCW16442 | Xanthine dehydrogenase | Sphingobium japonicum | 1.2E+00 | None | Amino acid metabolism |
| 15573 | 94585 | JAC34970 | Putative myosin class II heavy chain | Amblyomma triste | 7E-27 | Myosin_head | Skeletal muscle contraction |
| 04953 | 85346 | JAA60593 | Putative 24 kDa family member | Rhipicephalus pulchellus | 4E-38 | None | Unknown |
| 27862 | 82021 | JAB83083 | Putative myosin class I heavy chain | Ixodes ricinus | 3E-33 | Myosin_head | Skeletal muscle contraction |
| 00452 | 80428 | ECC00524 | Myosin heavy chain, skeletal muscle or cardiac muscle | Ixodes scapularis | 3E-26 | Myosin_N | Skeletal muscle contraction |
| 00140 | 79270 | JAA57343 | Calcium transporting ATPase | Rhipicephalus pulchellus | 00E+00 | Cation ATPase_N | Organelle cation transporter |
| 00619 | 78855 | JAC31893 | Putative actin binding cytoskeleton protein filamin | Amblyomma triste | 00E+00 | CH, filamin | Cytoskeleton |
| 51899 | 78108 | ETM32796 | Uncharacterized protein | Phytophthora parasitica | 8E+00 | None | Unknown |
| 08708 | 77869 | ELU14970 | Uncharacterized protein | Capitella teleta | 1E+00 | None | Unknown |
| 02127 | 75107 | JAC34859 | Putative mitogen inducible protein product | Amblyomma triste | 00E+00 | FERM_M | Unknown |
| 00589 | 74222 | JAA62349 | Uncharacterized protein | Rhipicephalus pulchellus | 1E-112 | None | Unknown |
| 00036 | 72607 | JAT93369 | Putative myosin class I heavy chain | Amblyomma aureolatum | 00E+00 | None | Skeletal muscle contraction |
| 00430 | 72504 | JAC31335 | Putative myosin regulatory light chain | Amblyomma triste | 1E-133 | EF-hand_6 | Skeletal muscle contraction |
| 00017 | 72240 | AAD17324 | Tropomyosin | Rhipicephalus microplus | 00E+00 | None | Skeletal muscle contraction |
| 00471 | 66789 | JAC94084 | Putative endocytosis/signaling protein | Ixodes ricinus | 00E+00 | None | Endocytosis |
| 73407 | 64871 | XP_657842 | Uncharacterized protein | Emericella nidulans | 2E-10 | None | Unknown |
| 00449 | 62738 | AEO34581 | Uncharacterized protein | Amblyomma maculatum | 00E+00 | None | Unknown |
| 00975 | 62079 | JAB81899 | Nucleolar GTP-binding protein | Ixodes ricinus | 00E+00 | NOGCT | Biogenesis of 60 s |
| 00477 | 61091 | JAC34490 | Putative alanine-glyoxylate aminotransferase agt2 | Amblyomma triste | 00E+00 | Aminotran_3 | Amino acid metabolism |
| 28761 | 60162 | EEC00415 | Putative ornithine decarboxylase | Ixodes scapularis | 9E-126 | Orn_Arg_deC_N, Orn_DAP_Arg_deC | Polyamine synthesis |
| 07560 | 60258 | JAC93558 | Uncharacterized protein | Ixodes ricinus | 1E-6 | None | Unknown |
| 00055 | 60152 | JAC22069 | Elongation factor 1-alpha | Amblyomma cajennense | 00E+00 | GTP_EFTU, GTP_EFTU_D2, GTP_EFTU_D3 | Elongation and nuclear export |
| 10926 | 60100 | JAC29751 | Putative alpha crystallins | Amblyomma triste | 5E-65 | HSP20 | Stress response |
| 01517 | 58463 | EEC19998 | Putative titin | Ixodes scapularis | 00E+00 | I-set, fn3 | Myofibril scaffold |
| 00690 | 55986 | EEC04237 | Putative cuticular protein | Ixodes scapularis | 3E-69 | CBM_14 | Chitin metabolism |
| 00310 | 55692 | JAA60289 | Eukaryotic translation initiation factor 4a2 | Rhipicephalus pulchellus | 00E+00 | DEAD | Translation initiation |
| 00960 | 55150 | EEC14479 | Putative stearoyl-CoA desaturase | Ixodes scapularis | 00E+00 | FA_desaturase | Iron binding/fatty acid metabolism |
| 17549 | 54635 | AAF81900 | Beta-actin | Aspergillus terreus | 2E-121 | None | Cytoskeleton |
| 00233 | 54395 | JAC22444 | Putative myosin class II heavy chain | Amblyomma cajennense | 00E+00 | None | Skeletal muscle contraction |
| 00080 | 53133 | JAC30913 | Putative secreted protein | Amblyomma triste | 4E-17 | None | Unknown |
| 00060 | 52729 | JAC34970 | Putative myosin class II heavy chain | Amblyomma triste | 00E+00 | Myosin_head | Skeletal muscle contraction |
| 00355 | 52661 | JAA61741 | Putative eukaryotic translation initiation factor 4g2 | Rhipicephalus pulchellus | 00E+00 | MIF4G | Translation initiation |
| 43456 | 51562 | EKN64830 | Uncharacterized protein | Bacillus azotoformans | 2E-1 | None | Unknown |
| 03024 | 50391 | JAC22994 | Putative secreted protein | Amblyomma cajennense | 3E-9 | None | Unknown |
| 00517 | 50298 | JAA60286 | Putative amb caj-77 translation factor | Rhipicephalus pulchellus | 00E+00 | None | Translation |
| 37644 | 50066 | JAA56007 | Putative der and -36 heat shock-related protein | Rhipicephalus pulchellus | 9E-75 | None | Stress response |
| 00954 | 49578 | JAC26141 | Putative enolase | Amblyomma parvum | 00E+00 | Enolase_C, enolase_N | Glycolysis |
| 02045 | 49068 | JAA60432 | Putative tick thioester protein | Rhipicephalus pulchellus | 00E+00 | A2M_comp, thiol-ester_cl | Fatty acid metabolism |
| 02199 | 48695 | JAA59973 | Putative ATP | Rhipicephalus pulchellus | 00E+00 | ABC_tran, ABC_tran_2 | ATPase activity |
a A2M_comp, A-macroglobulin complement component; ABC_tran, ATP-binding cassette transporter; ABC_tran_2, ATP-binding cassette transporter 2; Aminotran_3, aminotransferase class III; Cation ATPase_N, cation transporter/ATPase, amino-terminus; CBM_14, chitin binding peritrophin-A domain; CH, calponin homology domain; COX2, cytochrome C oxidase subunit II periplasmic domain; DEAD, DEAD/DEAH box helicase; EF-hand_6, EF-hand domain; Encolase_C, enolase carboxyl-terminus TIM barrel domain; Enolose_N, enolase amino-terminus; FA_desaturase, fatty acid desaturase; FERM_M, 4.1 protein, ezrin, radixin, moesin central domain; Filamin, filamin repeat domain; Fn3, fibronectin type III; GTP_EFTU, elongation factor Tu GTP binding; GTP_EFTU_D2, elongation factor Tu domain 2; GTP_EFTU_D3, elongation factor Tu carboxyl-terminus; HSP20, heat shock protein 20/alpha crystallin family; I-set, immunoglobulin intermediate-set; Ins145_P3_rec, inositol 1,4,5-triphosphate/ryanodine receptor; MIF4G, middle domain of eukaryotic initiation factor 4G; MIR, protein mannosyltransferase; Myosin_head, myosin head motor domain; Myosin_N, myosin amino-terminus; NOGCT, nucleolar GTP-binding protein carboxyl-terminus; Orn_Arg_deC_N, pyridoxal-dependent decarboxylase, pyridoxal binding domain amino-terminus; Orn_DAP_Arg_deC, pyridoxal-dependent decarboxylase carboxyl-terminus sheet domain; PDZ, post synaptic density protein; Thiol-ester_cl, alpha-macro-globulin thiol-ester bond forming region; Troponin, troponin.