Table 4.
Putative transcripts involved in chemoreceptor signal modulation/termination and xenobiotic metabolism identified exclusively in the Haller’s organ spf transcriptome of unfed, virgin adult male Dermacentor variabilis and their respective tick, nematode and insect matches with the lowest expect value (e-value). GenBank accession numbers are listed in Appendix A.
| Odorant Degradation Enzymes, ODE | Protein | Contig (Length, bp) | Top Tick Hit a %Identity & e-Value | Top C. elegans Hit b %Identity & e-Value | Top Insect Hit c %Identity & e-Value | Contig Conserved Domain(s) d |
|---|---|---|---|---|---|---|
 |
ODE, Cytochrome P450 | 69591 (575) | I. scapularis 57% & 1.30E-69 | C. elegans 43% & 2.00E-39 | D. mojavensis 42% & 1.30E-48 | p450 |
| 1691 (1001) | R. pulchellus 90% & 4.80E-79 | C. elegans 30% & 7.00E-7 | C. biroi 32% & 2.70E-9 | p450 | ||
| 6898 (1170) | I. scapularis 64% & 6.10E-123 | C. elegans 33% & 3.00E-30 | L. bostrychophila 40% & 3.0E-54 | p450 | ||
| 14383 (1167) | A. triste 69% & 2.30E-157 | C. elegans 31% & 2.00E-27 | B. tabaci 33% & 2.70E-41 | p450 | ||
| ODE, Glutathione S-transferase | 12057 (902) | A. triste 74% & 5.00E-110 | C. elegans 31% & 6.00E-7 | A. glabripennis 34% & 7.80E-38 | GST_C_3, GST_N | |
| 4931 (2250) | A. triste 88% & 3.30E-140 | C. elegans 30% & 7.00E-18 | A. rosae 31% & 1.00E-13 | GST_C, GST_N | ||
| ODE, Superoxide dismutase | 83534 (332) | R. pulchellus 70%, 2.20E-38 | C. elegans 55%, 3.00E-26 | P. cochleariae 65% & 4.90E-36 | Sod_Cu |
a Amblyomma triste, the hard tick; Ixodes scapularis, the blacklegged tick; Rhipicephalus pulchellus, the zebra tick; b Caenorhabditis elegans, the roundworm; c Anoplophora glabripennis, the Asian long-horned beetle; Athalia rosae, the turnip sawfly; Bemisia tabaci, the silverleaf whitefly; Cerapachys biroi, the clonal raider ant; Drosophila mojavensis, the fruit fly; Liposcelis bostrychophila, the booklouse; Phaedon cochleariae, the mustard beetle; d GST_C, glutathione S-transferase C-terminal domain; GST_C_3, glutathione S-transferase C-terminal domain; GST_N, glutathione S-transferase N-terminal domain; P450, cytochrome p450 domain; Sod_Cu, copper/zinc superoxide dismutase.