Figure 4.

Rac1b and Rac1 expression in HMEC of different passage numbers. (a) qPCR-based measurement of Rac1b and Rac1 expression in P12 and P16 HMEC. Data are the mean ± SD of three experiments (n = 3) and those from P16 HMEC are expressed relative to those in P12 HMEC set arbitrarily to 1.0; (b) Immunoblot-based measurement of Rac1 in HMEC of different passage numbers as indicated. As control (Co), a lysate from the pancreatic cancer cell line Panc1 ectopically expressing Rac1b-HA was used. A densitometric analysis of underexposed replicas is shown below the blots. Data are representative of three experiments.