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. 2017 Jul 20;18(7):1577. doi: 10.3390/ijms18071577

Figure 2.

Figure 2

Alternariol (AOH) and alternariol monomethyl ether (AME) suppress lipopolysaccharide (LPS)-induced innate immunity. BEAS-2B airway epithelial cells (panels a, c, and d) and RAW 264.7 mouse macrophages (panel b) at a density of 5 × 105 cells/well were treated with 10 μM of AOH and 10 μM of AME in the presence and absence of 10 μg of LPS and incubated for 24 h under normal conditions at 37 °C, 5% CO2. Supernatants were subsequently analyzed using enzyme-linked immunosorbent assay (ELISA) (a) IL8 BEAS-2B cells, (b) IL6 in RAW264.7 cells, (c) IL6 BEAS-2B cells, and (d) CCL2/MCP-1 BEAS-2B cells. An * indicates p < 0.05 according to Student’s t-test when comparing AOH/AME + LPS to LPS-induced controls. DMSO, dimethyl sulfoxide.