Figure 3.

Airway epithelium treated with alternariol (AOH) and alternariol monomethyl ether (AME) results in the downregulation of LPS-induced mRNAs. BEAS-2B cells seeded at a density of 5 × 10⁵ cells/well were treated with 10 μM AOH and 10 μg LPS for 24 h. The resulting RNA was harvested and measured with quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Each graph here demonstrates the upregulation and downregulation (fold change) of gene expression by normalization with the control GAPDH. (a) IL8, (b) CCL2, (c) IL6, (d) Caspase 1, and (e) CYP1A1 fold change. An * indicates p < 0.05 according to Student’s t-test when comparing AOH to dimethyl sulfoxide (DMSO) control and AOH + LPS to LPS corresponding control.