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. 2017 Jul 20;18(7):1577. doi: 10.3390/ijms18071577

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RNA silencing of the aryl hydrocarbon receptor (AhR) gene followed by treatment with lipopolysaccharide (LPS) and alternariol (AOH) in BEAS-2B cells. Cells were seeded at a density of 150,000 cells/well. Cells were treated with AhR siRNA for 24 h twice to successfully knockdown AhR. (a) IL8, and (b) IL6 released upon treatment with 10 μM AOH and 10 μg LPS for 24 h as measured by enzyme-linked immunosorbent assay (ELISA). An * indicates p < 0.05 according to Student’s t-test for AOH treatments (Scr or SiRNA) compared to LPS (Scr) or LPS (siRNA) controls, respectively.