Table 1.
Phenotype and fatty acid characteristics of Cellvibrio species.
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| 16S rDNA similarity to PR1 (%) | 100 | 98.6 | 98 | 97.4 | 97.3 | 95.7 |
| G+C content (mol%) | 49.98 | 48 | 47.4–48.4 | 52.6 | 44.6 | 44.9 |
| Nitrate reduced to nitrite | + | + | + | − | + | + |
| Utilization of | ||||||
| Maltose | + | + | + | + | + | + |
| Arabinose | + | + | + | + | + | + |
| Mannose | − | + | + | + | − | − |
| N-Acetyl-D-glucosamine | + | + | − | + | + | + |
| Carboxymethyl cellulose | − | + | + | + | + | + |
| Agar | + | − | − | − | − | − |
| Chitin | + | − | − | − | + | + |
| Valine arylamidase | − | − | + | − | − | − |
| Acid phosphatase | + | + | + | − | − | − |
| β-Galactosidase | + | − | − | − | − | − |
| β-Glucosidase | + | + | + | + | + | − |
| α-Galactosidase | + | + | + | − | + | + |
| β-Glucuronidase | + | − | − | − | + | + |
| α-Fucosidase | + | − | − | − | − | − |
| Leucine arylamidase | − | + | + | + | + | + |
| Naphthol-AS-BI-phosphohydrolase | − | − | + | + | + | + |
| Growth at 4°C (14 days) | − | + | + | − | + | − |
| Growth at 37°C | + | − | − | − | − | + |
| Mucoid growth on TSA | − | − | − | + | − | + |
| Yellow pigment on TSA | + | − | + | − | − | + |
| Fatty acid: | ||||||
| 10:0 | 2.33 | 4.8–7.1 | 4.1–8.2 | 2.6–5.8 | 4.20 | 4.3–6.9 |
| 10:0 3-OH | 2.57 | 7.7–10.7 | 5.5–13.4 | 13.00 | 6.50 | 6.1–10.9 |
| 12:0 | 1.99 | 5.4–7.7 | 4.0–7.2 | tr | 4.40 | 4.4–5.8 |
| 11:0 3-OH | — | 0.0–1.1 | tr | tr | — | — |
| 12:0 2-OH | — | 3.3–5.4 | 2.8–6.0 | 7.8–11.0 | 3.60 | 2.6–3.9 |
| 12:1 3-OH | 4.06 | — | — | — | 1.30 | — |
| 12:0 3-OH | — | 1.0–1.5 | tr | tr | — | — |
| 14:0 | 1.97 | 1.6–2.5 | 1.2–2.4 | 1.3–1.7 | tr | 0.9–1.2 |
| 15:0 | — | 0.0–1.3 | tr | 0.9–1.9 | 2.20 | tr |
| 16:0 | 36.74 | 15.7–20.0 | 18.5–27.8 | 14.3–18.0 | 19.70 | 17.6–20.9 |
| 17:0 | 2.40 | 1.6–4.5 | 0.0–1.9 | 2.2–3.7 | 5.70 | 1.3–2.0 |
| 17:1ω8c | — | tr | — | tr | tr | tr |
| 18:0 | 8.78 | 0.0–2.8 | 0.0–1.6 | tr | 3.10 | tr |
| 18:1ω6c | — | — | 0.0–2.8 | 0.0–3.3 | — | 0.0–4.3 |
| 18:1ω7c | — | 11.4–19.0 | 7.3–15.3 | 11.3–17.9 | 16.80 | 12.9–19.8 |
| 18:1ω9c | 2.87 | — | — | — | — | — |
| 19:0 | 2.90 | — | — | — | — | — |
| 20:0 | 6.81 | — | — | — | — | — |
| ECL 11.799 | — | — | — | — | — | tr |
| ECL 18.814 | — | tr | — | — | — | — |
| Summed feature 2 | 2.47 | — | — | — | — | — |
| Summed feature 3 | 17.37 | 25.6–34.0 | 34.4–38.1 | 30.4–34.9 | 30.90 | 34.1–38.2 |
| Summed feature 8 | 6.75 | — | — | — | — | — |
Strains: 1, PR1; 2, Cellvibrio fibrivorans LMG 18561T; 3, Cellvibrio ostraviensis LMG 19434T; 4, Cellvibrio mixtus ACM 2601T; 5, Cellvibrio fulvus LMG 2847T; 6, Cellvibrio vulgaris NCIMB 8633T. —, not detected; tr, trace (≤1.0% of total). Unknown fatty acids are designated by their equivalent chain-length (ECL), relative to the chain lengths of known saturated fatty acids. Summed feature 2 comprises iso-C16:1 I, C14:0 3-OH, and/or an unknown fatty acid. Summed feature 3 comprises C15:0 iso 2-OH, C16:1ω7c, or both. Summed feature 8 comprises C18:1ω7c and/or C18:1ω6c. Data for related Cellvibrio species came from Humphry et al. [22] and Mergaert et al. [23].