. Author manuscript; available in PMC: 2017 Nov 24.

Published in final edited form as: Nature. 2017 May 24;546(7658):401–405. doi: 10.1038/nature22400

Extended Data Table 2.

Validation of sequencing results.

Sample	Amplicon method	NGS platform	Mismatches/nucleotides covered^a
Sample	Amplicon method	NGS platform	3′UTR	CDS	5′ UTR
FL01M	35 × 400 bp	Ion S5	1/80	0/10272	7/252
	75 × ~200 bp^b	Ion S5	2/75	0/10272	4/205
	5 × ~2,200 bp	MiSeq	0/80	0/10272	0/32
FL03M	35 × 400 bp	Ion S5	3/87	0/10272	20/252
	75 × ~200 bp^b	Ion S5	4/78	0/10272	5/198
	5 × ~2,200 bp	MiSeq	0/82	0/10272	0/32

Compared to the consensus genomes generated by sequencing 35 × 400 bp amplicons on the MiSeq.

Amplicons produced using Ion AmpliSeq and 875 custom ZIKV primers.

NGS, next-generation sequencing; UTR, untranslated region; CDS, coding sequence.