Skip to main content
. Author manuscript; available in PMC: 2018 Jun 16.
Published in final edited form as: ACS Chem Biol. 2017 May 9;12(6):1691–1702. doi: 10.1021/acschembio.7b00241

Figure 1.

Figure 1

Crosstalk between the citrullination activity of PADs 2 and 4 and the methyltransferase activity of the PRC2 complex on Histone H3. (A) Schematic representation of proposed crosstalk between H3R26Cit and H3K27Me3. (B) Recombinant histone H3 was citrullinated by 100 nM PAD2 or PAD4 for 1 h prior to the addition of 50 nM of the PRC2 complex with 15 μM SAM for 2 h. PRC2 activity was measured in a 14C radiolabeled PAGE assay. (C) Recombinant histone H3 was methylated by 50 nM of the PRC2 complex with 15 μM SAM for 2 h before the addition of 100 nM PAD2 or PAD4 for 30 minutes. Citrulline levels were measured using the citrulline-specific chemical probe (Rh-PG).