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. 2017 Jul 26;12:5331–5343. doi: 10.2147/IJN.S137245

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© 2017 Luo et al. This work is published and licensed by Dove Medical Press Limited

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Cellular internalization of FA-PEG-SS-PEI-SPION/siRNA polyplex.

Notes: (A) Prussian blue staining of SGC-7901 cells treated with FA-PEG-SS-PEI-SPION/siScr at Fe concentrations of 10, 20, and 40 μg/mL, magnification 200×; (B) confocal images of SGC-7901 cells treated with FA-PEG-SS-PEI-SPION/siScr-FAM and PEG-SS-PEI-SPION/siScr-FAM.

Abbreviations: FA, folic acid; PEG, polyethylene glycol; SS, disulfide; PEI, polyethylenimine; SPION, superparamagnetic iron oxide nanoparticle; siScr-FAM, scrambled siRNA conjugated with 5-carboxyfluorescein; DAPI, 4′,6-diamidino-2-phenylindole; PP, PEG-SS-PEI-SPIONs; FP, FA-PEG-SS-PEI-SPIONs.