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. 2017 Jul 31;12(7):e0179615. doi: 10.1371/journal.pone.0179615

Fig 1. Expression and localization of the Trypanosoma cruzi AP-1 complex γ subunit (TcAP1-γ).

Fig 1

The purified recombinant protein (histidine-tagged TcAP1-γ hinge domain) (A) and T. cruzi whole cell extracts (B) were resolved on 10% SDS-PAGE gels, transferred to nitrocellulose membranes and reacted with the anti-TcAP1-γ mAb 211.F7 (produced against the hinge region of TcAP1-γ). E: epimastigotes; MT: metacyclic trypomastigotes; A: amastigotes. An antiserum against the T. cruzi GAPDH was used as loading control. (C) Parasites were incubated with the 211.F7 mAb (1:80 dilution), followed by incubation with anti-mouse IgG conjugated to Alexa Fluor 488 (1:600 dilution). Nuclear (n) and kinetoplast (k) DNA were stained with Hoechst 33342. (a) Epimastigote and (b) metacyclic trypomastigote. TcAP1-γ labelling is indicated by arrows. DIC, differential interference contrast microscopy. Scale bar = 5μm.