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. 2017 Jul 31;12(7):e0179615. doi: 10.1371/journal.pone.0179615

Fig 2. Immunofluorescence localization of the Trypanosoma cruzi AP-1 complex γ subunit (TcAP1-γ) in intracellular amastigotes.

Fig 2

T. cruzi-infected VERO cells were labelled with the anti-TcAP1-γ mAb 211.F7, which was detected with anti-mouse IgG conjugated to Alexa Fluor 488. (A) Labeling for TcAP1-γ (Alexa Fluor 488). (B) Host cell and parasite DNA stained with Hoechst 33342, detecting both the nuclear (n) and the kinetoplast (k) DNA of amastigotes. (C) Overlay of A and B, showing the localization of TcAP1-γ (arrow) near the kinetoplast (k). (D) DIC, differential interference contrast microscopy. Scale bar = 10μm.