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. 2017 Jul 31;12(7):e0179615. doi: 10.1371/journal.pone.0179615

Fig 3. Co-localization of Trypanosoma cruzi AP-1 complex γ subunit (TcAP1-γ) with T. cruzi Golgi markers.

Fig 3

Epimastigotes expressing the tagged Golgi marker Huntingtin interacting protein TcHIP-A/C (A-D) or tagged clathrin light chain TcCLC-A/C (E-H) were labelled with an anti-TcAP1-γ mAb 211.F7 that was detected with anti-mouse IgG conjugated to Alexa Fluor 488 followed by incubation with rabbit IgG anti-protein A for TcHIP-A/C and TcCLC-A/C, that were detected with anti-rabbit IgG conjugated to Alexa Fluor 546. Nuclear (n) and kinetoplast (k) DNA were stained with Hoechst 33342. Arrows indicate co-localization. DIC, differential interference contrast microscopy. Scale bar = 5μm.