Skip to main content
. 2017 Jul 31;12(7):e0179615. doi: 10.1371/journal.pone.0179615

Table 1. Primer sequences used in this work.

PRIMERS SEQUENCES (5’- 3’)
Hinge F GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGAAAATCGCCTCCAGGTATCC
Hinge R GGGGACCACTTTGTACAAGAAAGCTGGGTCCTAACAATCAAACACACATGCACTTCAGG
UPS_KpnI GTGGGTACCGTGTGTTGTGTTGTCCCTTTTTTTTTGTG
UPS_SalI ACCGTCGACCGGCAACGATCAAGTCACGAAGTCTCGCA
DOWN_BamHI GCGTGTGTGTGTGTGGATCCCTTTGTCGTCACGGAGAGAA
DOWN_XbaI CGCCAGCACAATCTAGACATAGCTAGACTGAAACT
AP-1-γ_attB1 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGAGGGGAAACTTGAGTCG
AP-1-γ_attB2 GGGGACCACTTTGTACAAGAAAGCTGGGTCCAACTCCTGCGTCACCTGA

attB1 and attB2 recombination sequences (for use with the Gateway cloning system) are shown in bold. Restriction sites are underlined.