Table 1. Primer sequences used in this work.
PRIMERS | SEQUENCES (5’- 3’) |
---|---|
Hinge F | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGAAAATCGCCTCCAGGTATCC |
Hinge R | GGGGACCACTTTGTACAAGAAAGCTGGGTCCTAACAATCAAACACACATGCACTTCAGG |
UPS_KpnI | GTGGGTACCGTGTGTTGTGTTGTCCCTTTTTTTTTGTG |
UPS_SalI | ACCGTCGACCGGCAACGATCAAGTCACGAAGTCTCGCA |
DOWN_BamHI | GCGTGTGTGTGTGTGGATCCCTTTGTCGTCACGGAGAGAA |
DOWN_XbaI | CGCCAGCACAATCTAGACATAGCTAGACTGAAACT |
AP-1-γ_attB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGAGGGGAAACTTGAGTCG |
AP-1-γ_attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTCCAACTCCTGCGTCACCTGA |
attB1 and attB2 recombination sequences (for use with the Gateway cloning system) are shown in bold. Restriction sites are underlined.