Fig 7. Forskolin prevents RSV-induced remodeling of the perijunctional actin cytoskeleton.

Confluent airway epithelial cell monolayers were either left untreated or infected with RSV for 48 h in the presence of either vehicle or forskolin (20 μM). Cells were fixed and labeled for F-actin or cortactin. Note that RSV infection caused the appearance of disorganized apical actin filaments and increased apical cortactin labeling (arrowheads). All these cytoskeletal alterations were attenuated by forskolin treatment (arrows). Scale bar, 40 μm. Image is representative of at least 3 independent experiments.