Fig 1. Characterisation of cpb-3(op234).

(A) Schematic of the CPB-3 protein, showing the position of its two RRMs and the C/H domain (ZnF). (B) Multiple sequence alignment of the ZnF motifs from clade-I CPEB homologs (see S1 Fig). Frequency corrected sequence logos [25] are shown above the alignment (cysteine and histidine amino acids are in red). Residue numbers at the bottom of the alignment refer to CPB-3; arrow indicates the position of the missense mutation (C520Y) in the cpb-3(op234) allele. Identical amino acids are highlighted in blue for more than 50% sequence identity, and in purple for more than 80% sequence identity. (C and D) Loss of cpb-3, but not other CPEB family members, leads to increased physiological germ cell death. Data shown are average number of germ cell corpses ± SEM from three (panel C) and two (panel D) independent experiments, scored in animals of the indicated genotypes 30 h (45 h for cpb-2 due to slow growth) post L4/adult molt, with at least 15 gonads in each experiment. Note: germ cell corpse counting for panel C and D was performed by different investigators, data should thus be compared within each panel but not across panels.