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. 2017 Jul 31;12(7):e0182142. doi: 10.1371/journal.pone.0182142

Fig 3. Nicotine CPP is reduced in mice with vMB α4 deletion.

Fig 3

a) CPP schematic. Prior to a 5-day, biased CPP procedure to establish nicotine CPP, mice were mildly food-restricted and handled. Drug-free pre-test and post-test days flanked 3 consecutive conditioning days that consisted of morning and afternoon saline (SAL) and nicotine (NIC) conditioning sessions. b) Nicotine CPP in C57Bl/6 WT mice. Groups of WT mice were conditioned with saline (n = 12), 0.25 mg/kg NIC (n = 12), or 0.5 mg/kg NIC (n = 12) to validate our CPP assay and identify a dose to be used subsequently in α4-flox mice. Mean (± SEM) place preference score is shown for the three drug doses. p values are for Dunnett’s multiple comparisons test. c) Nicotine CPP in α4-flox mice. AAV-GFP or AAV-Cre-GFP vectors were infused into vMB of α4-flox mice (GFP(+), n = 10; Cre(+), n = 10), and mice were subsequently conditioned with 0.25 mg/kg NIC. Mean (± SEM) place preference score is shown for both groups. p value is for unpaired t-test. d) Mean (± SEM) locomotor activity during the pre-test and post-test is shown for α4-flox mice conditioned with NIC. e) Mean (± SEM) locomotor activity during conditioning sessions 1, 2, and 3 is shown for α4-flox mice conditioned with NIC.