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. 2017 Jul 26;10:1755–1767. doi: 10.2147/JPR.S137131

Figure 3.

Figure 3

Real-time fluorescence quantitative polymerase chain reaction results show the expression of IL-1β, IL-6 and TNF-α mRNA in cultured rat dorsal spinal cord microglia cells.

Notes: (A) ADPβs-induced increased expression of IL-1β mRNA was significantly inhibited after pretreatment with MRS2395 (10 μM, 20 min) or MRS2211 (10 μM, 20 min). **P<0.01 vs control; +P<0.05 and ++P<0.01 vs ADPβs. (B) ADPβs-induced increased expression of IL-6 mRNA was significantly inhibited after pretreatment with MRS2395 (10 μM, 20 min) or MRS2211 (10 μM, 20 min). **P<0.01 vs control; +P<0.05 and ++P<0.01 vs ADPβs. (C) ADPβs-induced increased expression of TNF-α mRNA was significantly inhibited after pretreatment with MRS2395 (10 μM, 20 min) or MRS2211 (10 μM, 20 min). **P<0.01 vs control; +P<0.05 and ++P<0.01 vs ADPβs. (D) ADPβs (1, 10 and 50 μM) does not induce increased expression of IL-10 mRNA in microglia cells. All data were expressed as mean ± standard deviation of at least 3 independent experiments.

Abbreviations: IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α.