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. 2017 Jul 26;10:1755–1767. doi: 10.2147/JPR.S137131

Figure 6.

Figure 6

Enzyme-linked immunosorbent assay results show IL-1β, IL-6 and TNF-α release from cultured rat dorsal spinal cord microglia cells. Cells were incubated with the Y-27632 (20 μM, 1 h), SB203580 (40 μM, 2 h) or PDTC (40 μM, 4 h) before ADPβs (10 μM, 3 h) administration. After 3 h incubation, the culture supernatants were collected, and the amount of IL-1β, IL-6 and TNF-α were measured (A–C). (A) ADPβs induce IL-1β release from microglia cells (**P<0.01 vs control). ADPβs-induced IL-1β release was inhibited Y-27632 (#P<0.05 vs ADPβs), SB203580 (#P<0.05 vs ADPβs), or PDTC (##P<0.01 vs ADPβs). (B) ADPβs induce IL-6 release from microglia cells (**P<0.01 vs control). ADPβs-induced IL-6 release was significantly inhibited by Y-27632 (#P<0.05 vs ADPβs), SB203580 (#P<0.05 vs ADPβs), or PDTC (##P<0.01 vs ADPβs). (C) ADPβs induce TNF-α release from microglia cells (**P<0.01 vs control). ADPβs-induced TNF-α release was significantly inhibited by Y-27632 (#P<0.05 vs ADPβs), SB203580 (#P<0.05 vs ADPβs), or PDTC (##P<0.01 vs ADPβs). The data were the mean ± standard deviation of at least 3 independent experiments.

Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α.