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. 2017 Jul 31;12(7):e0182100. doi: 10.1371/journal.pone.0182100

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© 2017 Eneman et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — (A) Approximately 100 embryos exposed to adriamycin (0, 10 or 30 μM) in the swimming water (no injections), were live-screened at 4, 5 and 6 dpf and assigned to a phenotype category. (B) Approximately 100 embryos injected with PACAP morpholinos (adcyap1a and adcyap1b, 100 μM) were exposed to adriamycin (0, 10 or 30 μM) and then live-screened at 4, 5 and 6 dpf. PACAP morpholinos had a devastating effect on survival and morphology of the adriamycin exposed embryos. (C) Approximately 100 embryos injected with the human PACAP-38 peptide (5 μM) were exposed to adriamycin (0, 10 or 30 μM) and then live-screened at 4, 5 and 6 dpf. Human PACAP-38 could rescue the nephrotic phenotype in zebrafish with adriamycin exposure especially with the higher concentration (30 μM).