. Author manuscript; available in PMC: 2018 Feb 1.

Published in final edited form as: Protein Expr Purif. 2016 Oct 6;130:137–145. doi: 10.1016/j.pep.2016.10.001

Table 1.

Summary of purification data.

Gag Construct	Independent preparations	Yield mg/250 mL^a	Zn/protein	Mw SEC	c_1/2 (nM)^b 140 mM KCl	c_1/2 (nM)^b 340 mM KCl
Pr55^Gag	9	16 ± 2	1.8 ± 0.3	110 kDa	2.8 ± 0.2	22 ± 5
Pr55 ^Gag–His	6	12 ± 4	1.8 ± 0.4	112 kDa	1.1 ± 0.3	19 ± 3
Pr55 ^Gag–TEV-His	4	11 ± 4	1.7 ± 0.3	110 kDa	1.0 ± 0.1	21 ± 4
P55 ^Gag–ENLYFQ	8	6–10	1.5–0.2	107 kDa	2.9 ± 0.4	24 ± 3
Pr55^Gag Δp6	3	22 ± 4	ND	95 kDa	3.5 ± 0.5	21 ± 3
Pr55^Gag ΔSP2Δp6	3	21 ± 3	ND	95 kDa	ND	ND

ND Not determined.

Final yield of purified protein per 250 mL of ZYP5052.

Protein concentration at ½ maximal anisotropy. Average of titrations from at least three independent preparations of three technical replicates each.