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. Author manuscript; available in PMC: 2017 Oct 4.
Published in final edited form as: Cell Rep. 2016 Oct 4;17(2):609–623. doi: 10.1016/j.celrep.2016.09.021

Figure 2. Retinoic acid induces growth arrest and differentiation of neuroblastoma sphere-forming cells.

Figure 2

(A-B) Phase-contrast images (A) and quantification (B) of spheres treated with DMSO or 10 μM RA. Error bars (B) represent SD (n = 3).

(C) Growth assays of spheres treated with DMSO or 10 μM RA. Viable cells were quantified by trypan blue staining. Error bars represent SD (n = 4). Data were analyzed by two-way ANOVA with p values indicated.

(D-E) Tumor transplantation assay of spheres treated with DMSO or 10 μM RA for 7 days. Tumor weight (E) was analyzed by scatter plot with horizontal lines indicating the mean.

(F) qRT-PCR analysis of mRNA expression in spheres treated with DMSO or 10 μM RA. Error bars represent SD (n = 3).

Unless indicated, data were analyzed with two-tailed Student's t-test. ***p < 0.001. See also Figure S2.