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. 2017 Jul 17;13(7):e1006910. doi: 10.1371/journal.pgen.1006910

Fig 5. Abd-A and Hth function enhance oenocyte formation in both a cell-autonomous and non-cell-autonomous manner.

Fig 5

Fig 5A: Lateral view of the thoracic and first abdominal segments of a PrdG4;UAS-Rho embryo (stage 11) immunostained for phospho-ERK. The PrdG4-active second thoracic (T2) and first abdominal (A1) segments are labeled. Fig 5B: Quantitation of phospho-ERK expression in the PrdG4+ thoracic and abdominal segments of PrdG4;UAS-Rho embryos reveals equal activity between segments. Fig 5C-5F: Lateral views of wild type (C), PrdG4;UAS-AbdA (D), PrdG4;UAS-Rho (E), and PrdG4;UAS-Rho;UAS-AbdA (F) embryos (stage 15) immunostained for HNF4 (blue). The PrdG4+ thoracic (T2) and abdominal (A1) segments are labeled. Note, oenocyte numbers in wild type animals can vary from four to ten per abdominal segment [21, 24, 26]. Quantitative assessment of significant changes in oenocyte numbers are reported in Fig 5I. Fig 5G: Lateral view of hthp2;PrdG4;UAS-Rho embryo (stage 15) immunostained for HNF4 (blue). The PrdG4+ thoracic (T2) and abdominal (A1) segments are labeled. Fig 5H: Lateral view of PrdG4;UAS-PntP1 embryo (stage 15) immunostained for HNF4 (blue). The PrdG4+ thoracic (T2) and abdominal (A1) segments are labeled. Fig 5I: Quantitation of thoracic and abdominal oenocyte number per segment of at least 8 embryos from each genetic background. Red bars represent number of oenocytes per PrdG4+ abdominal segment, whereas blue bars represent number of oenocytes per PrdG4+ thoracic segment. * denotes p-value<0.01 and ** denotes p-value<0.05 for thoracic and abdominal segments.