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. 2017 Jul 17;13(7):e1006872. doi: 10.1371/journal.pgen.1006872

Fig 3. Open chromatin at the chr 12p12.1 RCC susceptibility locus overlaps with HIF-binding in primary renal tubular cells.

Fig 3

A) HIF-1α ChIP-seq track for PTC isolated from individual #1 indicates binding of HIF at the chr 12p12.1 region in PTC (blue). HIF-1α was stabilized by 1 mM DMOG for 4h. FAIRE-seq data from primary renal tubular cells from three individuals (PTC#1–3, red) and ENCODE FAIRE-seq tracks of 786-O RCC and a selection of non-renal cells. Enhancer associated SNPs, which are in high LD with the tag SNP rs718314 are indicated below the tracks. B) FAIRE qPCR experiments confirm chromatin accessibility at the rs12814794 associated enhancer specifically in renal tubule derived cells (RCC and PTC) when compared to non-renal cells. Data is from one experiment per cell line. Average enrichment values compared to a putative non-enhancer region (control locus) are shown.