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. 2017 Jul 17;13(7):e1006872. doi: 10.1371/journal.pgen.1006872

Fig 6. Interaction between HIF level, rs12814794 genotype and expression of BHLHE41 mRNA.

Fig 6

A) 33 genotyped primary tubular cell cultures (3 GG, 12 AG, and 18 AA) were exposed to 1 mM DMOG to induce HIF. BHLHE41 mRNA expression was assayed by qPCR. The mean (± standard deviation) inducibility of BHLHE41 mRNA compared to the respective untreated cells (= 1) was then plotted for each of the different rs12814794 genotypes. *, p<0.05—Student’s t-test. B) The mean inducibility of EGLN3 mRNA levels in the same samples was plotted as control. C-F) Box-and-whisker plots showing the correlation between BHLHE41 mRNA levels and genotype at rs12814794 in TCGA analysis of ccRCC tumors (KIRC). Tumors were first divided into tertiles according to expression of previously described HIF-1 or HIF-2 metagenes C) lower tertile for HIF-1 metagene expression, D) upper tertile for HIF-1 metagene expression, E) lower tertile for HIF-2 metagene expression, F) upper tertile for HIF-2 metagene expression. χ2-test was used to calculate p values for higher expression in GG individuals compared to AA individuals. Whiskers extend to 1.5 times of the inter quartile range.