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EMSA with either the duplex (Upper) or the template strand (Lower) cut back. The ovals indicate the terminus of the probe. The average binding is: 7/7, 40%; 8/8, 20%; 9/9 and 12/12, <2%, and for 1/1 probes (not shown), 50%. The average binding is: 7/12, 70%; 8/12, 20%; 9/12 and 11/12, <2%, and for 1/12 probes (not shown), 60%.
