Figure 2.

CPT inhibits the phosphorylation of STAT3 Tyr705 (pTyr705) in human gastric cancer cells. (a) The human gastric cancer cell lines SGC7901, MKN45, and HGC27 were incubated with 15 μM CPT for 4 h, and the levels of STAT3 pTyr705 and pSer727 were determined using western blotting with β-actin (ACTB) as an internal reference. (b) SGC7901 and MKN45 cells were treated with the indicated concentrations of CPT for 4 h. (c) SGC7901 or MKN45 cells were treated with 15 μM CPT for the indicated times. After treatment, western blotting analysis was used to determine the levels of STAT3 pTyr705 and STAT3 pSer727.