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. 2017 Mar 21;45(2):556–569. doi: 10.1177/0300060517692147

Figure 5.

Figure 5.

RAW264.7 cells transduced with β-catenin (encoded by catenin beta 1 [CTNNB1] gene) or controls were infected with Pseudomonas aeruginosa at a multiplicity of infection value of 1 and showed that β-catenin suppressed P. aeruginosa-triggered macrophage autophagy: (a) Representative Western blots showing microtubule-associated protein 1 light chain 3 alpha (LC3)-I, LC3-II and β-catenin protein levels with or without P. aeruginosa infection for 1, 2, 4 or 8 h (LC3-II:β-actin ratio shown underneath); (b) LC3 relative integrated density values normalized to β-actin, presented as ratio of control transduced cells showing lower LC3 levels in β-catenin versus control cells (*P < 0.05 at 2 h; ***P < 0.001 at 1 h, 4 h and 8 h); (c) Representative fluorescence micrograph showing endogenous LC3 stained cells following P. aeruginosa infection for 4 or 8 h; and (d) LC3 puncta quantified as the mean number of puncta/cell out of 100 cells per sample showing that stable overproduction of β-catenin reduced LC3 puncta formation (***P < 0.001 at 4 h and 8 h). Data presented as mean ± SEM of the mean for triplicate experiments. PA inf., P. aeruginosa infection duration; Ctl, control; β-cat, β-catenin.