Figure 3.

Western blot and reverse transcription–polymerase chain reaction (RT–PCR) analyses to investigate the effects of pretreatment with 50 mg/kg epigallocatechin-3-gallate (EGCG) prior to hepatic ischaemia–reperfusion injury on antioxidant enzyme protein and mRNA levels in mice. The three treatment groups were as follows: sham-operated group (Sham, n = 10), hepatic ischaemia–reperfusion injury group (IR, n = 10), and EGCG with ischaemia–reperfusion injury group (EGCG-treated IR, n = 10). (a) Representative Western blots showing oxidative stress markers, lipid peroxidation markers, and carbonyl reductase 1 (CBR1) down-stream enzymes after hepatic ischaemia–reperfusion injury. β-actin was used as a loading control; (b) Results of RT–PCR analysis of the mRNA levels of oxidative stress markers. The control housekeeping gene was glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Data are presented as mean ± SD. P < 0.05, one-way analysis of variance followed by Bonferroni’s multiple comparison test. HO-1, heme oxygenase-1; TRXr-1, thioredoxin reductase-1; NOS-2, nitric oxide synthase-2; Hsp70, heat shock protein 70; Hsp60, heat shock protein 60; HNE, hydroxynonenal; MDA, malondialdehyde; ALDH, aldehyde dehydrogenase; AR, aldose reductase.