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. Author manuscript; available in PMC: 2018 Jun 19.
Published in final edited form as: Dev Cell. 2017 Jun 19;41(6):638–651.e5. doi: 10.1016/j.devcel.2017.05.022

Figure 5. Chromosome mis-segregation triggers the evolution of complex abnormal karyotypes.

Figure 5

(A, B) Cells were grown as described in Figure 4A. After RO-3306 wash-out, cells were released into fresh medium and fixed 6 hours later to analyze 53BP1 foci in the following G1. Representative images are shown. 53BP1 is in red, γ-H2AX in green, CREST in magenta, DNA in blue. Quantification of 53BP1 foci in G1 is shown in (B). Scale bar 5 μm.

(C, D) Karyotype analysis after the first and second cell cycle following chromosome mis-segregation (see Figure S5 for experimental details). The percentage of cells with more than 2 chromosome changes (C) and the total number of chromosomal changes per cell (D) are shown for aneuploid cells after the first and second cell cycle following chromosome mis-segregation. *: p value < 0.05, Student’s t test.

See also Figure S5