Skip to main content
. Author manuscript; available in PMC: 2018 Jun 20.
Published in final edited form as: J Genet Genomics. 2017 Jun 13;44(6):319–326. doi: 10.1016/j.jgg.2017.05.006

Fig. 4. Thirty minutes of feeding after starvation acutely increases sleep.

Fig. 4

A: Schematic of experimental setup. One group of adult flies “fed ad lib” were placed into Drosophila Activity Monitor tubes on 5% sucrose and 2% agar and fed ad libitum throughout the experiment. One group of adult flies “starved > fed” was starved on 1% agar for 24 h and then transferred back onto 5% sucrose and 2% agar. One group of adult flies “starved > fed 30 min” were starved on 1% agar for 23.5 h, allowed to feed on 5% sucrose and 2% agar for 30 min, and transferred back onto 1% agar. Sleep changes were measured for 4 h after starved flies were allowed to feed for 30 min. B: Sleep profile of fed ad lib (n = 15), starved > fed (n = 30), or starved > fed 30 min (n = 31) yw females. C: Quantification of sleep for 1 h in fed ad lib (n = 89), starved > fed (n = 59), or starved > fed 30 min (n = 101) yw flies. Sleep was increased in starved > fed and starved > fed 30 min yw flies compared to fed ad lib (P < 0.0001). D: Quantification of sleep for 4 h in fed ad lib (n = 89), starved > fed (n = 59), or starved > fed 30 min (n = 101) yw flies. Sleep was increased in starved > fed yw flies compared to fed ad lib (P < 0.0001), but decreased in starved > fed 30 min yw flies compared to fed ad lib (P < 0.0001).