Fig. 7.

Altered vascular patterning results in reduced feto-placental perfusion at sites of constricted fetal capillaries in Egfl7^−/− placentas. Microangiography of placentas from E12.5 C57BL/6J and Egfl7^−/− mice was performed using tomato-lectin (red). Representative images of 100 µm vibratome cross-sections of E12.5 placentas from C57BL/6J (+/+; A) and Egfl7^−/− (B) mice. Insets: higher magnification of representative images showing areas of reduced feto-placental perfusion in Egfl7^−/− mice. (C-H) Three-dimensional reconstruction of z-stack confocal images of placentas perfused with tomato-lectin (red) and subsequently immunostained with CD31 (green). Arrows indicate narrowed fetal capillaries and areas where a reduction in perfusion begins. Scale bars: 100 µm in A,B.