Fig. 10.

Wee1 regulates plasmatocyte differentiation through Tig expression. Confocal images of PLs from mid/late 3rd instar larva. All PLs contained P[Hml-Gal4] and P[UAS-GFP] with or without P[UAS-Wee] and Tig mutant alleles. (A-D) The CZ (Hml>GFP⁺) of Hml>Wee1 PLs had few plasmatocytes (GFP⁺ P1⁺) and many IPs (GFP⁺ low/no P1). (E-L) The CZ of Tig mutant PLs, with or without Wee1 overexpression, had high levels of mature plasmatocytes and few IPs. See Table 3 for quantification. (M-T) PLs containing P[Hml-Gal4] and P[UAS-GFP] with or without P[UAS-Wee1]. Wee1 induced strong activation of Tig protein (compare M with O) in 50% of PLs examined (n=12). For Tig-lacZ, strong Wee1-dependent activation of the reporter (compare Q with R) was observed in 75% of PLs (n=12). For minR-lacZ, strong induction (compare S with T) was observed in all PLs (n=10). All animals were reared at 29°C. Scale bars: 50 µm.