Fig. 3.

Spatiotemporal analysis reveals dynamic patterns of acinar and duct markers during lacrimal gland development. (A-E) E16 to adult lacrimal glands were immunostained for acinar and ductal markers. SOX10 was readily detected in acinar cells by P1, but slowly declined at later stages of lacrimal gland development. (F-J) AQP5 expression increased at postnatal stages, was prominent in acinar cells before P4, and was apically restricted in acini and small ducts by P7. (K-O) MIST1 was expressed in some acinar cells by P4, and in most acinar cells by P7 and adulthood. (P-T) KRT19 marked ductal cells at all time points evaluated. (U-Y) NKCC1 was broadly expressed at E16, but switched between ductal and acinar compartments between P1 and P7. By adulthood, it was specifically expressed in ducts. Asterisks mark acini and arrowheads mark ducts. Scale bars: 100 μm.