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. 2017 Jul 1;144(13):2352–2363. doi: 10.1242/dev.151613

Fig. 4.

Fig. 4.

AGM CAM recipients harbor long-term multilineage reconstitution. (A-C) Bright filter (BF) and fluorescent (GFP) pictures of spleen (A), thymus (B) and bursa of Fabricius (C) collected from an adult wild-type chicken (gray background) and a reconstituted AGM CAM recipient (green background) at 5 months post-transplantation. Pictures show the whole organ. An enlarged view of the boxed region is shown below each picture. Flow cytometry analysis showing donor-cell contribution (GFP) in the spleen, thymus and bursa of Fabricius of the AGM CAM recipient (right panels in A-C, respectively). Cells were stained using anti-Bu-1 (B cells), anti-CD3 or anti-CD4 and anti-CD8 (T cells) antibodies. (D) Flow cytometry analysis showing donor-cell contribution (GFP+) in the BM of the AGM CAM recipient. Cells were stained using anti-CD41 (thrombocytes), anti-KUL01 (monocytes/macrophages), anti-Kit (hematopoietic stem/progenitor cells), anti-CD3 (T cells) and anti-Bu-1 (B cells) antibody. (E) May-Grünwald Giemsa staining of sorted GFPlow and GFPhigh cells collected from the blood of a reconstituted AGM CAM recipient at 5 months post-transplantation. Scale bars: 5 mm in A-C (top pictures); 1 mm in A-C (bottom pictures); 8 µm in E.