Fig. 2.

The OreR nuclear genotype contributes to a temperature-sensitive ovarian failure that is enhanced by simw⁵⁰¹ mitochondria. Ovarioles labeled with DAPI from different mito-nuclear females at day 3 of adulthood raised at either 25°C (A-D) or 28°C (E-H). The germarium (G) and stages (S) of egg chamber maturation are indicated. Scale bars: 50 µm. (I,J) Quantification of the percentage of germaria with different numbers of germline stem cells (GSCs) from the indicated mito-nuclear females raised at either 25°C (I) or 28°C (J). The numbers on the bars represent the total number of germaria analyzed. Comparison of (ore); OreR and (simw⁵⁰¹); OreR at 28°C, ***P<0.001 for two GSC, **P<0.01 for zero GSC, ns, non-significant for one GSC. Data are mean±s.e.m. (K-M) Germaria from (simw⁵⁰¹); OreR females raised at 28°C with two (K), one (L) or zero (M) GSCs, labeled with antibodies against Hts (red) and Vasa (green) proteins, and DAPI (blue). Solid outlines: GSCs with Hts-labeled spherical spectrosomes. Dotted outlines: niche positions without a GSC. Scale bars: 20 µm. (N-P) Germline cell death in germaria from (simw⁵⁰¹); OreR females raised at 28°C, labeled with antibodies against Hts (red) and cleaved caspase Dcp-1 (green). Images show germaria with no Dcp-1 labeling (N), a Dcp-1 labeled GSC (arrow) (O) or a Dcp-1 labeled 16 cell cyst (P). Scale bars: 10 µm.