Fig. 3.

A temperature-sensitive mito-nuclear incompatibility in the mother severely reduces embryonic hatch rate. (A-D) Embryonic hatch rate depends on the temperature of the (simw⁵⁰¹); OreR mother. G0 mothers were raised at 25°C or 28°C and their G1 embryos allowed to develop at the same or reciprocal temperature. Embryonic hatch rates were measured for mothers of different ages post eclosion (x axis). Data are mean percentage of G1 hatched eggs±s.e.m. for three biological replicates. (A) Mothers at 25°C and embryos at 25°C. (B) Mothers at 28°C and embryos 28°C. (C) Mothers at 25°C and embryos at 28°C. (D) Mothers at 28°C and embryos 25°C. ***P<0.001 comparing (ore); OreR and (simw⁵⁰¹); OreR. n=3. (E) The compatible Aatm^A allele in the mother rescues embryonic hatch rate. Hatch rates of embryos were measured from mothers with different mitochondria and doses of nuclear Aatm alleles, as indicated below the x axis. Mothers were at 28°C and embryos at 25°C. n=3 biological replicates; data are mean±s.e.m. (***P<0.001). See Fig. S5 for cross scheme.