(A) Immunofluorescent staining results showed the increased number of caspase-8, NLRP3 or ASC positive cells with decreased number of NLRP6 positive cells in HCECs at 450mOsM, which was completely reversed by 20μM C8-Inh, but partly by P3-Inh. (B) Double Immunofluorescent staining showed that caspase-8 involved in NLRP3/ASC/caspase-8 inflammasomes in HCECs exposed to 450mOsM medium, which was completely reversed by 20μM C8-Inh, but partly by P3-Inh. Data shown are representative of three or more independent experiments.