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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Autoimmun. 2017 Feb 24;80:65–76. doi: 10.1016/j.jaut.2017.02.006

Fig. 6. Imbalanced activation of NLRP3/NLRP6 inflammasomes triggers inflammation in ocular surface of experimental mice under desiccating stress.

Fig. 6

(A and B) Immunofluorescent staining and RT-qPCR showed stimulated immunoreactivity and/or mRNA of NLRP3 and ASC with decreased NLRP6 in corneal and conjunctival epithelia of desiccating (DS) mice with untreated (UT) mice as controls. (C) Western blotting showed the imbalanced activation between NLRP3/ASC and NLRP6 as well as caspase-1 activation in corneal and conjunctival epithelia of DS mice compared with UT mice. (D and E) Activity of caspase-1 and caspase-8 was significantly increased in corneal and conjunctival epithelia of DS mice, evaluated by immunofluorescent staining and activity assay. (F) Immunofluorescent staining showed enhanced production of BRCC3, IL-1β and IL-18 in ocular surface epithelia of DS mice. Data shown are representative of three or more independent experiments (mean ± SD). *P < 0.05, **P < 0.01, vs. controls.