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. 2001 Jul 24;98(16):9038–9043. doi: 10.1073/pnas.161278998

Figure 1.

Figure 1

Analysis of cross-linked SplB by SDS/PAGE. (10His)SplB was treated as described in Experimental Procedures, denatured, and electrophoresed through 10% SDS/PAGE. (A) Coomassie blue-stained gel. (B) Western blot. Lanes 1 and 4, untreated; lanes 2 and 5, cross-linked with DMS; lanes 3 and 6, cross-linked with DMS after incubation under conditions favoring [Fe-S] cluster formation. Filled circles indicate positions of cross-linked species at a molecular mass of ≈64 kDa and ≈72 kDa. The lane marked M contains molecular weight markers. Sizes are in kilodaltons.