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Analysis of (10His)SplB by size exclusion chromatography. (10His)SplB was chromatographed through Biogel P-100, immediately after its purification by Ni-NTA chromatography (A) and after incubation under conditions to reconstitute [Fe-S] clusters (B). The y axes (A280) in A and B are scaled identically. (C) Results of colorimetric iron assays (16) performed on fractions from the experiment depicted in B.
