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EPR analysis of (10His)SplB. EPR spectra of (10His)SplB isolated under aerobic conditions (protein concentration 6 mg/ml; A); (10His)SplB treated with 10 mM dithionite (protein concentration 12 mg/ml; B); and (10His)SplB treated with 10 mM dithionite and 1 mM S-AdoMet (protein concentration 12 mg/ml; C). In all three traces, relative signal strength (y axes) are depicted on identical scales.
