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. 2017 Jul 1;10(7):881–896. doi: 10.1242/dmm.028910

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Pulsed SILAC analysis of protein synthesis during atrophy. (A) Relative Lys6 incorporation for control muscle over time. The percentage labelling was calculated as (H/L)/(H/L+1). The right panel in A shows the AUC for all time points. (B) Relative Lys6 incorporation for denervated muscle over time. (C) Ratio of Lys6 incorporation in control and denervated muscles. Log₂ transformation ratios indicated a normal distribution. (D) One-dimensional enrichment and boxplot analysis show reduced Lys6 labelling of GO terms containing metabolic and myosin filament proteins (red boxes). Proteasomal proteins (blue boxes) exhibited increased Lys6 labelling. (E-G) Volcano plots of Lys6 incorporation in denervated and control muscles. One-sample t-test showing that significantly regulated proteins were observed only on day 7 (q<0.05). Day 4 samples were analysed as biological duplicates and day 7-11 as biological triplicates.