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. 2017 Jul 1;10(7):897–907. doi: 10.1242/dmm.029280

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Characterization of TPP1F-deficient cells. (A) Mutant 1-13 and 1-20 do not express the ∼65 kDa protein as revealed by analysis of whole-cell lysates (5×10⁵ cell equivalents per lane) with mAb K88-254-3 (TPP1F). Loading was assessed using cap34-specific mAb 135-409. (B) TPP1 activity is not impaired in growing mutant cells. Lysates from equal numbers of cells were used in the TPP1 assay. The data are the mean±s.d. from four independent experiments. The results from AX2 were set to 100%. The differences were not statistically significant.