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. 2017 Jul 15;130(14):2277–2291. doi: 10.1242/jcs.192781

Fig. 3.

Fig. 3.

LIPUS increases the number of early endosomes in a Rac1-dependent manner. (A) LIPUS stimulation in serum-starved B16 GFP-actin cells leads to the formation of actin comet-tails (arrowheads). The inset is shown in higher magnification in the right-hand panels at the indicated times (min). Data are mean±s.e.m. from three independent movies; n=17. Scale bar: 20 µm. (B) Immunofluorescence images (colour inverted) of B16 cells with or without LIPUS stimulation, stained for actin or EEA1. Scale bar: 20 µm. (C) Quantification of the number of endosomes in B16 control cells or cells expressing WT Rac1 or DN Rac1. Note that the expression of DN Rac1 blocks the LIPUS-induced increase in early endosomes. (D) Expression of CA Rac1 also prevents any change in the number of EEA1-positive early endosomes after LIPUS stimulation. Data in C and D are from three independent experiments and are presented as described in the Materials and Methods. **P<0.01.